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Anti-c-Met Antibody (Cytoplasmic Domain) IHC-plus™ LS-B2812

Note: This antibody replaces LS-C16508


Wt. Vol. Conc. Price
- 50 µl 1.14 mg/ml $395

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Mouse Monoclonal (IgG2a) to Human c-Met
IHC - Paraffin, IHC - Frozen


Human c-Met
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal


IHC - Paraffin (10 µg/ml), IHC - Frozen (1:50)

Specificity and Use

c-Met antibody was raised against synthetic peptide derived from cytoplasmic domain of human c-Met protein.
Cytoplasmic Domain
Recognizes c-Met.
Immunohistochemistry: LS-B2812 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2812 was determined to be 10 ug/ml. Positive control: Breast cancer.


PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About c-Met

P08581 NM_000245 NP_000236.2

MET Antibody, AUTS9 Antibody, C-Met Antibody, HGF/SF receptor Antibody, HGFR Antibody, Met kinase Antibody, Proto-oncogene c-Met Antibody, Scatter factor receptor Antibody, SF receptor Antibody, HGF receptor Antibody, RCCP2 Antibody, Tyrosine-protein kinase Met Antibody

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules.


Anti-c-Met antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2812 concentration 10 ug/ml.

Requested From: United States
Date Requested: 11/25/2015

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