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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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c‑Maf Antibody (aa324‑373) LS‑C112332
C-Maf antibody LS-C112332 is an unconjugated rabbit polyclonal antibody to c-Maf from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C112332-50
50 µg
Unavailable

Popular c-Maf Products

Anti-MAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1809 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-MAF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4581 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot
Antibody
Species: Mouse
Applications: Western blot, ELISA
Detection of Mouse cMaf by Western Blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with empty vector or expression constructs for mouse MafA, mouse MafB or mouse cMaf. Antibodies: Affinity purified rabbit anti-cMaf antibody used at 0.4 ug/ml. Detection: Chemiluminescence.
Species: Mouse, Bovine
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Chromatin Immunoprecipitation

Product Description

C-Maf antibody LS-C112332 is an unconjugated rabbit polyclonal antibody to c-Maf from human, mouse, rat and other species. Validated for WB.
About c-Maf
Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. O75444 NM_005360 NP_005351.2

MAF Antibody, C-MAF Antibody, CCA4 Antibody, C-maf proto-oncogene Antibody, Proto-oncogene c-Maf Antibody, T lymphocyte c-maf long form Antibody, Transcription factor Maf Antibody


Specifications

Target
Human c-Maf
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
c-Maf antibody was raised against synthetic peptide located between aa324-373 of human MAF (O75444-1, NP_005351). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Opossum, Guinea pig, Zebra finch, Chicken, Zebrafish (100%); Xenopus (92%); Platypus (85%).
Blocking Peptide
LS-E10776 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112332. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa324-373
Specificity
Human MAF
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MAF antibody LS-C112332 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAF antibody LS-C112332 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAF antibody LS-C112332 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAF antibody LS-C112332 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAF antibody LS-C112332 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAF antibody LS-C112332 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

MAF antibody LS-C112332 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAF antibody LS-C112332 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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