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Anti-C/EBP Beta / CEBPB Antibody (clone 47A1) IHC-plus™ LS-B2926

Note: This antibody replaces LS-C93711

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular C/EBP Beta / CEBPB Antibodies

Anti-C/EBP Beta / CEBPB Antibody (aa39-78, clone 21A1) IHC-plus™ LS-B1688
Mouse Monoclonal [clone 21A1] (IgG) to Human C/EBP Beta / CEBPB
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-C/EBP Beta / CEBPB Antibody (clone 47A1) IHC-plus™ LS-B2926
Mouse Monoclonal [clone 47A1] (IgG1,k) to Human C/EBP Beta / CEBPB
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 47A1] (IgG1,k) to Human C/EBP Beta / CEBPB
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human C/EBP Beta / CEBPB
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [47A1]
Unconjugated
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA

Specificity and Use

C/EBP Beta / CEBPB antibody was raised against recombinant human C/EBP-kappa(1-271 aa) purified from E. coli.
Immunohistochemistry: LS-B2926 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2926 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4, 0.1 % sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About C/EBP Beta / CEBPB

P17676 NM_005194 NP_005185.2

CEBPB Antibody, C/EBP-beta Antibody, C/EBP beta Antibody, CRP2 Antibody, LAP Antibody, Liver activator protein Antibody, Nuclear factor NF-IL6 Antibody, IL6DBP Antibody, TCF5 Antibody, NFIL6 Antibody, TCF-5 Antibody, Transcription factor 5 Antibody, NF-IL6 Antibody

Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Binds to regulatory regions of several acute-phase and cytokines genes and probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Plays an important role in adipose tissue differentiation.

Immunohistochemistry

Immunohistochemistry
Anti-CEBPB / CEBP Beta antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2926 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CEBPB / CEBP Beta antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2926 concentration 10 ug/ml.

Western blot

Western blot
NIH 3T3 cell lysates(30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-beta antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/10/2016

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