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BRUNOL5 / CELF5 Antibody (aa323‑372) LS‑C116187 Best Seller
CELF5 antibody LS-C116187 is an unconjugated rabbit polyclonal antibody to CELF5 (BRUNOL5) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C116187-50
50 µg
Unavailable

Popular BRUNOL5 / CELF5 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

CELF5 antibody LS-C116187 is an unconjugated rabbit polyclonal antibody to CELF5 (BRUNOL5) from human, mouse, rat and other species. Validated for WB.
About BRUNOL5 / CELF5
RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Q8N6W0 NM_021938 NP_068757.2

CELF5 Antibody, Brunol5 Antibody, CELF-5 Antibody, Bruno-like protein 5 Antibody, CUG-BP and ETR-3 like factor 5 Antibody, BRUNOL-5 Antibody, RNA-binding protein BRUNOL-5 Antibody


Specifications

Target
Human BRUNOL5 / CELF5
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
BRUNOL5 / CELF5 antibody was raised against synthetic peptide located between aa323-372 of human CELF5 (Q8N6W0, NP_068757). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%); Orangutan, Elephant, Bat, Rabbit, Opossum, Zebra finch, Chicken, Xenopus (84%).
Blocking Peptide
LS-E15029 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116187. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa323-372
Specificity
Human CELF5 / BRUNOL5
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

CELF5 / BRUNOL5 antibody LS-C116187 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CELF5 / BRUNOL5 antibody LS-C116187 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CELF5 / BRUNOL5 antibody LS-C116187 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CELF5 / BRUNOL5 antibody LS-C116187 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CELF5 / BRUNOL5 antibody LS-C116187 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CELF5 / BRUNOL5 antibody LS-C116187 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CELF5 / BRUNOL5 antibody LS-C116187 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CELF5 / BRUNOL5 antibody LS-C116187 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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