BRCA1 antibody LS-B3772 is an unconjugated rabbit polyclonal antibody to BRCA1 from human, mouse and rat. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot
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BRCA1 antibody was raised against synthetic peptide surrounding amino acid 1852 of human BRCA1.
Immunohistochemistry: LS-B3772 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3772 was determined to be 5 ug/ml.
PBS, pH 7.2, 0.01% Thimerosal, 30% Glycerol, 0.5% BSA
Short term: -20°C; Long term: -70°C.
For research use only.