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Anti-BRCA1 Antibody IHC-plus™ LS-B3772

Note: This antibody replaces LS-C48349

Ordering

Wt. Vol. Conc. Price
50 µg - 0.2 mg/ml Unavailable

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Anti-BRCA1 Antibody (C-Terminus, clone GLK-2) LS-C95602
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Anti-BRCA1 Antibody IHC-plus™ LS-B3772
Rabbit Polyclonal to Human BRCA1
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Human BRCA1
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human BRCA1
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • Immunoprecipitation

Specificity and Use

BRCA1 antibody was raised against synthetic peptide surrounding amino acid 1852 of human BRCA1.
Immunohistochemistry: LS-B3772 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3772 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.2, 30% glycerol, 0.5% BSA, 0.01% Thimerosal.
Long term: -70°C; Short term: -20°C
For research use only.

About BRCA1

P38398 NM_007294 NP_009225.1

BRCA1 Antibody, BROVCA1 Antibody, BRCC1 Antibody, Breast cancer 1, early onset Antibody, PPP1R53 Antibody, PSCP Antibody, PNCA4 Antibody, RING finger protein 53 Antibody, RNF53 Antibody, BRCAI Antibody, Breast cancer 1 early onset Antibody, IRIS Antibody

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function.

Immunohistochemistry

Immunohistochemistry
Anti-BRCA1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3772 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-BRCA1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3772 concentration 5 ug/ml.

Requested From: 
Date Requested: 4/27/2017

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