Clostridium botulinum Botulinum Neurotoxin Type A
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
Synthetic peptide analogue of the carboxyl terminal region of C. botulinum Toxin A.
Recognizes and binds to intact whole C. botulinum Toxin A, amino acids 1280-1292. Has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of C. botulinum Toxin A. Inhibits the entry of C. botulinum Toxin A to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin. Cross-reactivity: C. botulinum Toxin A (1280-1292) and C. botulinum Toxin A: 100%, C. botulinum Toxin B, C, E and F (1272-1280): 0%.
Suitable for use in ELISA, Immunofluorescence and RIA. Immunocytochemistry1:50. Note: Prior incubation of the antibody with synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding by the antibody.
No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.