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Anti-BMI1 / PCGF4 Antibody (Internal, clone EPR3745(2)) IHC-plus™ LS-B8010

Note: This antibody replaces LS-C152396

Ordering

Wt. Vol. Conc. Price
- 50 µl - Unavailable

Most Popular BMI1 / PCGF4 Antibodies

Anti-BMI1 / PCGF4 Antibody (aa252-264) IHC-plus™ LS-B346
Goat Polyclonal to Human BMI1 / PCGF4
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BMI1 / PCGF4 Antibody (clone 229F6) LS-C150548
Mouse-Balb/c Monoclonal [clone 229F6] (IgG1)
Human, Mouse, Rabbit
IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Anti-BMI1 / PCGF4 Antibody (Internal, clone EPR3745(2)) IHC-plus™ LS-B8010
Rabbit Monoclonal [clone EPR3745(2)] (IgG) to Human BMI1 / PCGF4
Human, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Monoclonal [clone EPR3745(2)] (IgG) to Human BMI1 / PCGF4
Human, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human BMI1 / PCGF4
Rabbit
Human, Rat (tested or 100% immunogen sequence identity)
IgG Monoclonal [EPR3745(2)]
Unconjugated
Tissue culture supernatant
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • ICC (1:100 - 1:250)
  • Immunofluorescence (1:250)
  • Western blot (1:10000 - 1:50000)

Specificity and Use

BMI1 / PCGF4 antibody was raised against a synthetic peptide corresponding to residues in human BMI-1.
Internal
Human BMI-1. Species cross-reactivity is based on WB analysis.

Packaging

Tris-Glycine-NaCl or PBS, pH 7.2-7.4, 40-50% Glycerol, 0.01% sodium azide, 0.05% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About BMI1 / PCGF4

P35226 NM_005180 NP_005171.4

BMI1 Antibody, BMI-1 Antibody, Flvi-2/bmi-1 Antibody, FLVI2/BMI1 Antibody, PCGF4 Antibody, Polycomb group protein Bmi1 Antibody, Polycomb complex protein BMI-1 Antibody, Dna-binding protein bmi-1 Antibody, Polycomb group ring finger 4 Antibody, RING finger protein 51 Antibody, RNF51 Antibody

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Immunohistochemistry

Immunohistochemistry
Anti-BMI-1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8010 dilution 1:50.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of SW480 cells using anti-BMI-1 antibody.

Western blot

Western blot
Western blot analysis on (A) K562, (B) Saos-2, (C) SW480, (D) Molt-4, and (E) HT-1080 cell lysates using anti-BMI-1 antibody.
 
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Requested From: 
Date Requested: 3/25/2017

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