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Anti-BMI1 / PCGF4 Antibody (aa96-326) LS-C149862

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BMI1 / PCGF4 Antibodies

Anti-BMI1 / PCGF4 Antibody (aa252-264) IHC-plus™ LS-B346
Goat Polyclonal to Human BMI1 / PCGF4
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BMI1 / PCGF4 Antibody (clone 229F6) LS-C150548
Mouse-Balb/c Monoclonal [clone 229F6] (IgG1)
Human, Mouse, Rabbit
IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Anti-BMI1 / PCGF4 Antibody (Internal, clone EPR3745(2)) IHC-plus™ LS-B8010
Rabbit Monoclonal [clone EPR3745(2)] (IgG) to Human BMI1 / PCGF4
Human, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human BMI1 / PCGF4
Human
ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human BMI1 / PCGF4
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Flow Cytometry

Specificity and Use

BMI1 / PCGF4 antibody was raised against e. coli-derived recombinant human BMI1 Asp96-Gly326 Accession # P35226.
aa96-326
Detects human BMI1 in direct ELISAs and Western blots.
Western Blot, 0.1 µg/ml, Recombinant Human BMI-1. Immunocytochemistry, 5-15 µg/ml, Intracellular Staining by Flow Cytometry, 2.5 µg/106 cells, HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and. permeabilized with saponin.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About BMI1 / PCGF4

P35226 NM_005180 NP_005171.4

BMI1 Antibody, BMI-1 Antibody, Flvi-2/bmi-1 Antibody, FLVI2/BMI1 Antibody, PCGF4 Antibody, Polycomb group protein Bmi1 Antibody, Polycomb complex protein BMI-1 Antibody, Dna-binding protein bmi-1 Antibody, Polycomb group ring finger 4 Antibody, RING finger protein 51 Antibody, RNF51 Antibody

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Immunocytochemistry

Immunocytochemistry
BMI-1 in K562 Human Cell Line. BM I-1 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using 10 ug/ml Goat Anti-Human BMI-1 Antigen Affinity-purified Polyclonal Antibody for 3 hours at room temperature. Cells were stained with the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
 

Requested From: United States
Date Requested: 12/2/2016

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