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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BLK Antibody (aa1‑30) LS‑C99166
BLK antibody LS-C99166 is an unconjugated rabbit polyclonal antibody to BLK from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C99166-400
400 µl
Unavailable

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Product Description

BLK antibody LS-C99166 is an unconjugated rabbit polyclonal antibody to BLK from human and mouse. Validated for IHC and WB.
About BLK
Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. P51451 NM_001715 NP_001706.2

BLK Antibody, Blk kinase Antibody, B lymphocyte kinase Antibody, B lymphoid tyrosine kinase Antibody, MODY11 Antibody, Protein kinase blk Antibody, Protein tyrosine kinase blk Antibody, B lymphoid kinase Antibody, p55-Blk Antibody, Tyrosine-protein kinase Blk Antibody


Specifications

Target
Human BLK
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9205 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99166. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-30
Specificity
This BLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BLK.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

BLK Antibody (G1) western blot of HL-60 cell line lysates (35 ug/lane). The BLK antibody detected the BLK protein (arrow).
BLK Antibody (G1) western blot of HL-60 cell line lysates (35 ug/lane). The BLK antibody detected the BLK protein (arrow).

Western blot

Western blot of anti-BLK antibody transiently transfected HEK-293 cell line lysate (1 ug/lane). BLK antibody(arrow) was detected using the purified antibody.
Western blot of anti-BLK antibody transiently transfected HEK-293 cell line lysate (1 ug/lane). BLK antibody(arrow) was detected using the purified antibody.

Western blot

Western blot of anti-BLK antibody in mouse kidney tissue lysate. BLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of anti-BLK antibody in mouse kidney tissue lysate. BLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

BLK Antibody (G1) western blot of HL-60 cell line lysates (35 ug/lane). The BLK antibody detected the BLK protein (arrow).
BLK Antibody (G1) western blot of HL-60 cell line lysates (35 ug/lane). The BLK antibody detected the BLK protein (arrow).

Western blot

Western blot of anti-BLK antibody transiently transfected HEK-293 cell line lysate (1 ug/lane). BLK antibody(arrow) was detected using the purified antibody.
Western blot of anti-BLK antibody transiently transfected HEK-293 cell line lysate (1 ug/lane). BLK antibody(arrow) was detected using the purified antibody.

Western blot

Western blot of anti-BLK antibody in mouse kidney tissue lysate. BLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of anti-BLK antibody in mouse kidney tissue lysate. BLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

BLK Antibody (G1) western blot of HL-60 cell line lysates (35 ug/lane). The BLK antibody detected the BLK protein (arrow).
BLK Antibody (G1) western blot of HL-60 cell line lysates (35 ug/lane). The BLK antibody detected the BLK protein (arrow).

Western blot

Western blot of anti-BLK antibody transiently transfected HEK-293 cell line lysate (1 ug/lane). BLK antibody(arrow) was detected using the purified antibody.
Western blot of anti-BLK antibody transiently transfected HEK-293 cell line lysate (1 ug/lane). BLK antibody(arrow) was detected using the purified antibody.

Western blot

Western blot of anti-BLK antibody in mouse kidney tissue lysate. BLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of anti-BLK antibody in mouse kidney tissue lysate. BLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

BLK Antibody (G1) western blot of HL-60 cell line lysates (35 ug/lane). The BLK antibody detected the BLK protein (arrow).
BLK Antibody (G1) western blot of HL-60 cell line lysates (35 ug/lane). The BLK antibody detected the BLK protein (arrow).

Western blot

Western blot of anti-BLK antibody transiently transfected HEK-293 cell line lysate (1 ug/lane). BLK antibody(arrow) was detected using the purified antibody.
Western blot of anti-BLK antibody transiently transfected HEK-293 cell line lysate (1 ug/lane). BLK antibody(arrow) was detected using the purified antibody.

Western blot

Western blot of anti-BLK antibody in mouse kidney tissue lysate. BLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot of anti-BLK antibody in mouse kidney tissue lysate. BLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.


Requested From: 
Date Requested: 6/23/2018

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