Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Delipidated and defibrinated
Western blot (1:1000)
ELISA (1:5000 - 1:25000)
Specificity and Use
BIVM peptide corresponding to a region near carboxy terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: C-R-N-S-G-Y-Q-G-Y-S-D-Y-D-G-N-D.
This polyclonal antibody reacts human BIVM at the C- terminal region and is useful for detection of BIVM by ELISA or western blotting. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A 57 kD band corresponding to human BIVM is detected. Unstimulated HeLa cells can be used as a positive control.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below.
Western blot of anti-human BIVM. Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody. A 57 kDa band corresponding to human BIVM protein is detected using a 1:1000 dilution of the antiserum incubated for 1 hour at room temperature. Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) LS-C60865 diluted 1:10000 and incubated for 1 hour at room temperature. The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure). Other detection methods will yield similar results.
Western blot of anti-human BIVM. Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody. A 57 kD band corresponding to human BIVM protein is detected using a 1:1000 dilution of the antiserum.