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Anti-BIRC5 / Survivin Antibody (clone 5B10) LS-C196668

Catalog Size Price
LS-C196668-100 100 µg Unavailable

Most Popular BIRC5 / Survivin Antibodies

Anti-BIRC5 / Survivin Antibody LS-C26299
Mouse Monoclonal (IgG1,k) to Human BIRC5 / Survivin
Human, Rat
Immunofluorescence
Unconjugated
Anti-BIRC5 / Survivin Antibody LS-C26302
Mouse Monoclonal (IgG2a,k) to Human BIRC5 / Survivin
Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-BIRC5 / Survivin Antibody (N-Terminus) LS-C26305
Mouse Monoclonal (IgG1) to Human BIRC5 / Survivin
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-BIRC5 / Survivin Antibody (Azide-free) LS-C88693
Rabbit Polyclonal (IgG) to Human BIRC5 / Survivin
Human, Mouse
IHC, IHC - Paraffin
Unconjugated, Azide-free
Formalin-fixed, paraffin-embedded human stomach stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells in the gastric glands.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-BIRC5 / Survivin Antibody (C-Terminus) IHC-plus™ LS-B2787
Rabbit Polyclonal to Human BIRC5 / Survivin
Human, Mouse
IHC, IHC - Paraffin
Unconjugated
Anti-BIRC5 / Survivin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2787 concentration 10 ug/ml.

100% Guaranteed
Mouse Monoclonal [clone 5B10] (IgG2a,k) to Human BIRC5 / Survivin
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA, RNA interference
Unconjugated

Details

Human BIRC5 / Survivin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [5B10]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
  • RNA interference

Specificity and Use

BIRC5 (NP_001159, aa1-100 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human BIRC5 / Survivin
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About BIRC5 / Survivin

O15392 NM_001168 NP_001159.2

BIRC5 Antibody, API4 Antibody, Apoptosis inhibitor survivin Antibody, Apoptosis inhibitor 4 Antibody, Survivin Antibody, Survivin variant 3 alpha Antibody, EPR-1 Antibody, IAP4 Antibody

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis.

Immunofluorescence

Immunofluorescence of monoclonal antibody to BIRC5 on HeLa cell . [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to BIRC5 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western Blot analysis of BIRC5 expression in transfected 293T cell line by BIRC5 monoclonal antibody (M01), clone 5B10.Lane 1: BIRC5 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of BIRC5 expression in transfected 293T cell line by BIRC5 monoclonal antibody (M01), clone 5B10.Lane 1: BIRC5 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation of BIRC5 transfected lysate using anti-BIRC5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BIRC5 rabbit polyclonal antibody.
Immunoprecipitation of BIRC5 transfected lysate using anti-BIRC5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BIRC5 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BIRC5 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BIRC5 is approximately 0.03 ng/ml as a capture antibody.

RNA interference

Western blot analysis of BIRC5 over-expressed 293 cell line, cotransfected with BIRC5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BIRC5 monoclonal antibody (M01), clone 5B10 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of BIRC5 over-expressed 293 cell line, cotransfected with BIRC5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BIRC5 monoclonal antibody (M01), clone 5B10 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: 
Date Requested: 5/22/2018

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