Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Xenopus (at least 90% immunogen sequence identity)
Western blot (0.3 - 1 µg/ml)
Specificity and Use
BEND5 antibody was raised against synthetic peptide CKNEERREAKYNLQ from the C-terminus of human BEND5 (NP_078879.2). Percent identity by BLAST analysis: Hamster, Elephant, Rabbit, Guinea pig, Platypus, Lizard (100%); Xenopus (93%).
LS-E28089 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186448. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kD band observed in Human Tonsil and Spleen and Mouse Spleen lysates and also in lysates of cell line Daudi (calculated MW of 48.2kD according to NP_078879.2). Recommended concentration: 0.3-1 ug/ml.
BEND5 antibody (0.3 ug/ml) staining of Daudi (A), Human Tonsil (B), Human Spleen (C) and Mouse Spleen (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.