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Anti-BCL2L2 / Bcl-w Antibody (clone 2E4) LS-C196738

Catalog Size Price
LS-C196738-100 100 µg Unavailable

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Immunohistochemical analysis of BCLW staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Western blot of recombinant BCL2L2 / BCL-W.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Mouse Monoclonal [clone 2E4] (IgG2a,k) to Human BCL2L2 / Bcl-w
Human
Western blot, ELISA
Unconjugated

Details

Human BCL2L2 / Bcl-w
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2E4]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

BCL2L2 / Bcl-w antibody was raised against bCL2L2 (NP_004041.1, aa1-193 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human BCL2L2 / BCL-W
Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About BCL2L2 / Bcl-w

Q92843 NM_004050 NP_004041.1

BCL2L2 Antibody, BCLW Antibody, BCL2-L-2 Antibody, KIAA0271 Antibody, PPP1R51 Antibody, Apoptosis regulator Bcl-W Antibody, Bcl-2-like protein 2 Antibody, BCL-W Antibody, BCL2-like 2 Antibody

BCL2L2 / Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.

Western blot

BCL2L2 monoclonal antibody (M01), clone 2E4. Western Blot analysis of BCL2L2 expression in human colon.
BCL2L2 monoclonal antibody (M01), clone 2E4. Western Blot analysis of BCL2L2 expression in human colon.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BCL2L2 is 10 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BCL2L2 is 10 ng/ml as a capture antibody.

Requested From: 
Date Requested: 5/22/2018

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