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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCAT1 / ECA39 Antibody (aa81‑107) LS‑C156761
ECA39 antibody LS-C156761 is an unconjugated rabbit polyclonal antibody to human ECA39 (BCAT1). Validated for IHC and WB.
Catalog
Size
Price
LS-C156761-400
400 µl
Unavailable

Popular BCAT1 / ECA39 Products

BCAT1 antibody LS-C107998 Western blot of Fetal Small Intestine lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Bat, Dog, Rabbit
Applications: Western blot
Anti-BCAT1 / ECA39 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11204 concentration 10 ug/ml.
Species: Human, Mouse, Dog
Applications: IHC, IHC - Paraffin, Western blot
Western blot analysis of ECA39 expression in Jurkat (A); Ramos (B); NIH3T3 (C); PC12 (D) whole cell lysates.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep
Applications: Western blot
Immunohistochemistry of Human colon cancer using BCAT1 Polyclonal Antibody at dilution of 1:50.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Immunohistochemistry of Human cervical cancer using BCAT1 Polyclonal Antibody at dilution of 1:50.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ELISA

Product Description

ECA39 antibody LS-C156761 is an unconjugated rabbit polyclonal antibody to human ECA39 (BCAT1). Validated for IHC and WB.
About BCAT1 / ECA39
BCAT1 / ECA39 is the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. P54687 NM_005504 NP_005495.2

BCAT1 Antibody, BCAT(c) Antibody, BCATC Antibody, ECA39 Antibody, MECA39 Antibody, BCT1 Antibody, Placental protein 18 Antibody, PNAS121 Antibody, PP18 Antibody, Protein ECA39 Antibody


Specifications

Target
Human BCAT1 / ECA39
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E74 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156761. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa81-107
Specificity
This BCAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-107 amino acids from the Central region of human BCAT1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

BCAT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
BCAT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

BCAT1 Antibody western blot of Ramos cell line lysates (35 ug/lane). The BCAT1 antibody detected the BCAT1 protein (arrow).
BCAT1 Antibody western blot of Ramos cell line lysates (35 ug/lane). The BCAT1 antibody detected the BCAT1 protein (arrow).

Immunohistochemistry

BCAT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
BCAT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

BCAT1 Antibody western blot of Ramos cell line lysates (35 ug/lane). The BCAT1 antibody detected the BCAT1 protein (arrow).
BCAT1 Antibody western blot of Ramos cell line lysates (35 ug/lane). The BCAT1 antibody detected the BCAT1 protein (arrow).

Immunohistochemistry

BCAT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
BCAT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

BCAT1 Antibody western blot of Ramos cell line lysates (35 ug/lane). The BCAT1 antibody detected the BCAT1 protein (arrow).
BCAT1 Antibody western blot of Ramos cell line lysates (35 ug/lane). The BCAT1 antibody detected the BCAT1 protein (arrow).

Immunohistochemistry

BCAT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
BCAT1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

BCAT1 Antibody western blot of Ramos cell line lysates (35 ug/lane). The BCAT1 antibody detected the BCAT1 protein (arrow).
BCAT1 Antibody western blot of Ramos cell line lysates (35 ug/lane). The BCAT1 antibody detected the BCAT1 protein (arrow).


Requested From: 
Date Requested: 6/18/2018

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