Target
Human BCAN / Brevican
Synonyms
BCAN | Brevican | BEHAB | CSPG7 | Brevican core protein | BCAN1 | BCAN2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rhBrevican. (aa 23 - 911).
Specificity
Recognizes human Brevican.
Applications
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation (25 µg/ml)
- Functional Assay
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1-2 ug/ml detection limit for rhBrevican is ~10 ng/lane under non-reducing conditions. Immunoprecipitation: 25 ug/ml. Blocking of receptor-ligand interaction: ~0.01 to 0.05 ug/ml of this antibody will block 50% of the binding of 1 ug/ml of Hyaluronan (132kD) to immobilized rhBrevican (100 ul of a 3 ug/mL solution was coated in each well) in a functional ELISA. At 1 ug/ml, this antibody will achieve 100% blocking in the assay.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Lyophilized powder may be stored at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee