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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAR / BFAR Antibody (C‑Terminus) IHC‑plus™ LS‑B4629
Note: This antibody replaces LS-C108539
BFAR antibody LS-B4629 is an unconjugated rabbit polyclonal antibody to BFAR (BAR) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4629-50
50 µg (1 mg/ml)
Unavailable

Popular BAR / BFAR Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot
Immunoperoxidase of monoclonal antibody to BFAR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using LS-C148222 at 1:2000. A. Two cores from a human glioblastoma tissue microarray: 1 =fibrillary astrocytoma (grade I), and2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-Eos in counterstain.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Western blot analysis of BAR expression in A549 (A); SHSY5Y (B); mouse brain (C); mouse liver (D); rat heart (E) whole cell lysates.
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

BFAR antibody LS-B4629 is an unconjugated rabbit polyclonal antibody to BFAR (BAR) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About BAR / BFAR
Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors. Q9NZS9 NM_016561 NP_057645.1

BFAR Antibody, BAR Antibody, RNF47 Antibody, RING finger protein 47 Antibody


Specifications

Target
Human BAR / BFAR
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30857 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B4629. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
BFAR antibody can be used for detection of BFAR by Western blot at 1-2 ug/ml.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-BFAR antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4629 concentration 5 ug/ml.
Anti-BFAR antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4629 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 ug/ml.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 ug/ml.

Western blot

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-BFAR antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4629 concentration 5 ug/ml.
Anti-BFAR antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4629 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 ug/ml.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 ug/ml.

Western blot

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-BFAR antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4629 concentration 5 ug/ml.
Anti-BFAR antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4629 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 ug/ml.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 ug/ml.

Western blot

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-BFAR antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4629 concentration 5 ug/ml.
Anti-BFAR antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4629 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 ug/ml.
Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 ug/ml.

Western blot

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 ug/ml.


Requested From: 
Date Requested: 6/20/2018

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