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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAG3 / BAG‑3 Antibody (aa563‑575) IHC‑plus™ LS‑B3925
Note: This antibody replaces LS-C54565
BAG-3 antibody LS-B3925 is an unconjugated goat polyclonal antibody to human BAG-3 (BAG3). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3925-50
50 µg (0.5 mg/ml)
Unavailable

Popular BAG3 / BAG-3 Products

BAG3 antibody LS-C111174 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit
Applications: Western blot
Western blot of BAG-3 in CAPAN-1 human pancreatic cancer cells following heat shock using LS-C148217 at !:2000, Cells were heat shocked at 45 degrees for 10min and total proteins were extracted following incubation at 37 degrees for the indicated times. Control= no heat shock treatment. Maxi mal BAG-3 protein expression was detected at 6 h post heat shock treatment.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections using LS-C148218 at 1:2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-Eosin counterstain.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Anti-BAG3 / BAG-3 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10292 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

BAG-3 antibody LS-B3925 is an unconjugated goat polyclonal antibody to human BAG-3 (BAG3). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About BAG3 / BAG-3
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. O95817 NM_004281 NP_004272.2

BAG3 Antibody, Bcl-2-associated athanogene 3 Antibody, Bcl-2-binding protein Bis Antibody, BCL2-binding athanogene 3 Antibody, BIS Antibody, BAG-3 Antibody, CAIR-1 Antibody, Docking protein cair1 Antibody, BCL2-associated athanogene 3 Antibody, Docking protein CAIR-1 Antibody


Specifications

Target
Human BAG3 / BAG-3
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BAG3 / BAG-3 antibody was raised against synthetic peptide C-SSMTDTPGNPAAP from the C-terminus of human BAG3 (NP_004272.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (85%), Monkey (85%), Marmoset (85%).
Blocking Peptide
LS-E25374 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3925. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa563-575
Specificity
Human BAG3.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-BAG3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-BAG3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.
Anti-BAG3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3925 concentration 3.75 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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