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Anti-BAG3 / BAG-3 Antibody (aa537-548) IHC-plus™ LS-B10292

Note: This antibody replaces LS-C204974
See our new BAG3 / BAG-3 ELISA Kits
Human BAG3 / BAG-3 ELISA Kit - LS-F7573
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

BAG3 / BAG-3 Antibody LS-B10292 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-BAG3 / BAG-3 antibody IHC staining of human brain, cortex.
Western blot
BAG3 antibody (0.1 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer).

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Goat Polyclonal to Human BAG3 / BAG-3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human BAG3 / BAG-3
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.1 - 0.3 µg/ml), ELISA (1:16000)

Specificity and Use

BAG3 / BAG-3 antibody was raised against synthetic peptide C-KNAGNAEDPHTE from an internal region of human BAG3 / BAG-3 (NP_004272.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Monkey (92%).
aa537-548
Human BAG3 / BAG-3
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 70kD band observed in Human Pancreas lysates (calculated MW of 61.6kD according to NP_004272.2). Recommended concentration: 0.1-0.3 ug/ml.

Packaging

Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About BAG3 / BAG-3

O95817 NM_004281 NP_004272.2

BAG3 Antibody, Bcl-2-associated athanogene 3 Antibody, Bcl-2-binding protein Bis Antibody, BCL2-binding athanogene 3 Antibody, BIS Antibody, BAG-3 Antibody, CAIR-1 Antibody, Docking protein cair1 Antibody, BCL2-associated athanogene 3 Antibody, Docking protein CAIR-1 Antibody

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells.

Immunohistochemistry

Immunohistochemistry
Anti-BAG3 / BAG-3 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10292 concentration 5 ug/ml.

Western blot

Western blot
BAG3 antibody (0.1 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 7/31/2015

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