Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Mouse BAG1 / BAG-1
Mouse, Human (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
IHC - Paraffin (1:1000 - 1:5000)
IHC - Frozen
Western blot (1:1000 - 1:2000)
Immunoprecipitation (1:50 - 1:200)
Specificity and Use
BAG1 / BAG-1 antibody was raised against a synthetic peptide corresponding to amino acids 26-45 (NERYDLLVTPQQGNSEPVVQD) of mouse BAG-1, GenBank no. AAH03722.1. This sequence is 100% conserved in mouse BAG-1L; GenBank no. NP_033866.3. Percent identity by BLAST analysis: Mouse (100%); Rat (95%); Gorilla, Gibbon, Monkey (81%); Human (76%).
Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms.
Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.
Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.