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Anti-BAG1 / BAG-1 Antibody (aa26-45) LS-C148209

Catalog Size Price
LS-C148209-50 50 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Mouse BAG1 / BAG-1
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Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain. Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.
Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.

Details

Mouse BAG1 / BAG-1
Rabbit
Mouse, Human (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)

Specificity and Use

BAG1 / BAG-1 antibody was raised against a synthetic peptide corresponding to amino acids 26-45 (NERYDLLVTPQQGNSEPVVQD) of mouse BAG-1, GenBank no. AAH03722.1. This sequence is 100% conserved in mouse BAG-1L; GenBank no. NP_033866.3. Percent identity by BLAST analysis: Mouse (100%); Rat (95%); Gorilla, Gibbon, Monkey (81%); Human (76%).
aa26-45
BAG1.

Packaging

Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About BAG1 / BAG-1

NM_004323

BAG1 Antibody, Bcl-2-associated athanogene 1 Antibody, Bcl-2-binding protein Antibody, Bcl2 binding protein bag1 Antibody, BCL2-associated athanogene Antibody, BAG-1 Antibody, Bcl2 binding athanogene1 Antibody, HAP Antibody, RAP46 Antibody

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms.
(More About BAG1 / BAG-1)

Immunohistochemistry

Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.
Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.

Western blot

Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.
Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.
Requested From: 
Date Requested: 4/21/2018
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