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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAG1 / BAG‑1 Antibody (aa26‑45) LS‑C148209
BAG-1 antibody LS-C148209 is an unconjugated rabbit polyclonal antibody to BAG-1 (BAG1) from human and mouse. Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C148209-50
50 µl
Unavailable

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Formalin-fixed and paraffin-embedded human brain tissue reacted with BAG1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: ICC, Western blot, Flow Cytometry, ELISA

Product Description

BAG-1 antibody LS-C148209 is an unconjugated rabbit polyclonal antibody to BAG-1 (BAG1) from human and mouse. Validated for IHC, IP and WB.
About BAG1 / BAG-1
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. NM_004323

BAG1 Antibody, Bcl-2-associated athanogene 1 Antibody, Bcl-2-binding protein Antibody, Bcl2 binding protein bag1 Antibody, BCL2-associated athanogene Antibody, BAG-1 Antibody, Bcl2 binding athanogene1 Antibody, HAP Antibody, RAP46 Antibody


Specifications

Target
Mouse BAG1 / BAG-1
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BAG1 / BAG-1 antibody was raised against a synthetic peptide corresponding to amino acids 26-45 (NERYDLLVTPQQGNSEPVVQD) of mouse BAG-1, GenBank no. AAH03722.1. This sequence is 100% conserved in mouse BAG-1L; GenBank no. NP_033866.3. Percent identity by BLAST analysis: Mouse (100%); Rat (95%); Gorilla, Gibbon, Monkey (81%); Human (76%).
Epitope
aa26-45
Specificity
BAG1.
Presentation
Antiserum, 0.05% sodium azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.
Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.

Western blot

Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.
Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.

Immunohistochemistry

Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.
Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.

Western blot

Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.
Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.

Immunohistochemistry

Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.
Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.

Western blot

Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.
Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.

Immunohistochemistry

Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using LS-C148209 at 1:2000. A1 is a higher magnification of A. DifferentialBag1 expression levels are seen in the hypothalamic nuclei. The variation in expression levels from lack of Bag1 staining to strong staining may be linked to differential responses of the hypothalamic nuclei to ischemic stress. Hematoxylin-Eos in counterstain.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.
Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using LS-C148209 at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-Eosin counterstain.

Western blot

Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.
Western blot of BAG-1 in normal brain tissue lysates using LS-C148209 at 1:2000.


Requested From: 
Date Requested: 6/22/2018

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