Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone FMC7] (IgM) to Human B cell
Human B cell
Human (tested or 100% immunogen sequence identity)
IgM Monoclonal [FMC7]
Flow Cytometry (1:1)
Specificity and Use
B cell antibody was raised against hRIK cells - Human B-Lymphoblastoid line.
Recognizes a glycoprotein antigen of 105kD expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukemia and hairy cell leukemia. The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognize the CD22 molecule itself. A recent publication by Serke et al has shown Recognizes a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request.
0.1 M Tris-HCL, 0.5 ml sodium chloride, 10 mM glycine, pH 8.0, 0.09% sodium azide, 1% BSA.