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Anti-AURKB / Aurora-B Antibody (phospho-Thr232) IHC-plus™ LS-B22

Note: This antibody replaces LS-C18931

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AURKB / Aurora-B Antibodies

Anti-AURKB / Aurora-B Antibody (aa227-238) IHC-plus™ LS-B1451
Rabbit Polyclonal to Human AURKB / Aurora-B
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AURKB / Aurora-B Antibody (aa1-344) IHC-plus™ LS-B2882
Sheep Polyclonal to Human AURKB / Aurora-B
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human AURKB / Aurora-B
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human AURKB / Aurora-B
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:250 - 1:2000)
  • ELISA (1:10000 - 1:50000)

Specificity and Use

AURKB / Aurora-B antibody was raised against synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein.
pThr232
Immunohistochemistry: LS-B22 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B22 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About AURKB / Aurora-B

Q96GD4 NM_004217 NP_004208.2

AURKB Antibody, AIRK2 Antibody, AIK2 Antibody, Aurora kinase B Antibody, Aurora kinase B-Sv1 Antibody, Aurora-related kinase 2 Antibody, Aurora/IPL1-related kinase 2 Antibody, ARK-2 Antibody, ARK2 Antibody, Aurkb-sv1 Antibody, Aurkb-sv2 Antibody, Aurora 1 Antibody, Aurora-B Antibody, AIM-1 Antibody, IPL1 Antibody, STK-1 Antibody, STK5 Antibody, PPP1R48 Antibody, AurB Antibody, Aurora B Antibody, Aurora kinase B-Sv2 Antibody, Aurora-1 Antibody, Aurora1 Antibody, Serine/threonine kinase 12 Antibody, STK1 Antibody, STK12 Antibody

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis.

Immunohistochemistry

Immunohistochemistry
Anti-AURKB / Aurora-B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B22 concentration 5 ug/ml.

Western blot

Western blot
Anti-Aurora B pT232 antibody - Western Blot. Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500. Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs). Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen.

Requested From: United States
Date Requested: 12/3/2016

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