Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Human AURKB / Aurora-B
Human, Mouse (tested or 100% immunogen sequence identity)
IHC - Paraffin (2.5 µg/ml)
Western blot (1:250 - 1:2000)
ELISA (1:10000 - 1:50000)
Specificity and Use
AURKB / Aurora-B antibody was raised against synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein.
Immunohistochemistry: LS-B22 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B22 was determined to be 2.5 ug/ml.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis.
Anti-AURKB / Aurora-B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B22 concentration 5 ug/ml.
Anti-Aurora B pT232 antibody - Western Blot. Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500. Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs). Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen.
Requested From: United States
Date Requested: 12/3/2016