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Anti-AURKB / Aurora-B Antibody (aa1-344) IHC-plus™ LS-B2882

Note: This antibody replaces LS-C82240

Ordering

Wt. Vol. Conc. Price
- 50 µl 0.105 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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AURKB / Aurora-B Antibody LS-B2882 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-AURKB / Aurora-B antibody IHC of human tonsil.
Immunohistochemistry
Anti-AURKB / Aurora-B antibody IHC of human skin.

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Sheep Polyclonal to Human AURKB / Aurora-B
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human AURKB / Aurora-B
Sheep
Human (tested or 100% immunogen sequence identity)
Polyclonal
Affinity purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (0.2 µg/ml)

Specificity and Use

AURKB / Aurora-B antibody was raised against recombinant human His(6)-Aurora B [residues 1 - 344] expressed in insect cells.
aa1-344
Recognizes human Aurora B
Immunohistochemistry: LS-B2882 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2882 was determined to be 10 ug/ml.

Packaging

Purified antiserum, PBS, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About AURKB / Aurora-B

Q96GD4 NM_004217 NP_004208.2

AURKB Antibody, AIRK2 Antibody, AIK2 Antibody, Aurora kinase B Antibody, Aurora kinase B-Sv1 Antibody, Aurora-related kinase 2 Antibody, Aurora/IPL1-related kinase 2 Antibody, ARK-2 Antibody, ARK2 Antibody, Aurkb-sv1 Antibody, Aurkb-sv2 Antibody, Aurora 1 Antibody, Aurora-B Antibody, AIM-1 Antibody, IPL1 Antibody, STK-1 Antibody, STK5 Antibody, PPP1R48 Antibody, AurB Antibody, Aurora B Antibody, Aurora kinase B-Sv2 Antibody, Aurora-1 Antibody, Aurora1 Antibody, Serine/threonine kinase 12 Antibody, STK1 Antibody, STK12 Antibody

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis.

Immunohistochemistry

Immunohistochemistry
Anti-AURKB / Aurora-B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2882 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-AURKB / Aurora-B antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2882 concentration 10 ug/ml.

Requested From: United States
Date Requested: 8/1/2015

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