Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Immunofluorescence, Western blot, Immunoprecipitation
Human AURKB / Aurora-B
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Immunofluorescence (1:50), Western blot (1:10001), Immunoprecipitation
Specificity and Use
AURKB / Aurora-B antibody was raised against synthetic peptide corresponding to aa1-19 (KLH).
Recognizes human Aurora B. Species cross-reactivity: Rat and mouse.
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Immunofluorescence: 1:50. Western Blot: 1:10001:2000. Immunoprecipitation: 50-60 ug to immunoprecipitate Aurora B from a RIPA extract of 300 ug of human HeLa cells. Positive control: HeLa cell lysate.
PBS, pH 7.4, 1% BSA, 15 mM sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis.