Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3H1] (IgG1,k) to Human AURKA / Aurora-A
ICC, Immunofluorescence, Western blot
Human AURKA / Aurora-A
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3H1]
Protein G purified
ICC (1:500 - 1:1000)
Immunofluorescence (1:500 - 1:1000)
Western blot (1:1000)
Specificity and Use
AURKA / Aurora-A antibody was raised against full length recombinant human Aurora A expressed in and purified from E. coli.
Clone 3H1 recognizes Aurora A kinase specifically both in western blots and in immunocytochemical experiments. On blots, clone 3H1 reveals a prominent 46 kDa band, on cells in tissure cultures, clone 3H1 reveals strong staining in spindle poles and midbodies.
The antibody solution can be used at dilutions 1:500-1:1000 for immunofluorescence. For western blots try at 1:1000.
PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes.
Shows HeLa cell cultures stained with AURKA / Aurora-A antibody antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. Counterstained is a chicken polyclonal antibody against Vimentin (red). Blue is a DNA stain.
Western analysis of AURKA / Aurora-A antibody. Blot of HeLa cells treated with 100 ng/mL of nocodazole for 18 hours was probed with AURKA / Aurora-A antibody. Nocodazole is a microtubule inhibitor which induces cells G2/M phase and induces Aurora A expression. The AURKA / Aurora-A antibody monoclonal binds strongly and cleanly to a band at about 46 kDa.