Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk.
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
Anti-ATP5B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.
Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.