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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATP5B / ATP Synthase Beta Antibody (clone 7F4) LS‑C114921
ATP Synthase Beta antibody LS-C114921 is an unconjugated mouse monoclonal antibody to ATP Synthase Beta (ATP5B) from human, dog and monkey. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C114921-100
100 µl
Unavailable

Popular ATP5B / ATP Synthase Beta Products

ATP5B antibody LS-C81176 was used in IHC to stain formalin-fixed, paraffin-embedded human muscle.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot
Western blot Image
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Anti-ATP5B / ATP Synthase Beta antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Monkey, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

ATP Synthase Beta antibody LS-C114921 is an unconjugated mouse monoclonal antibody to ATP Synthase Beta (ATP5B) from human, dog and monkey. Validated for Flow, IF, IHC and WB.
About ATP5B / ATP Synthase Beta
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. P06576 NM_001686 NP_001677.2

ATP5B Antibody, ATPMB Antibody, ATPSB Antibody, Beta-mtATPase Antibody, F0F1-ATP synthase beta subunit Antibody, ATP synthase beta subunit Antibody


Specifications

Target
Human ATP5B / ATP Synthase Beta
Host
Mouse
Reactivity
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [7F4]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
Specificity
Human ATP5B
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-ATP5B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.
Anti-ATP5B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-ATP5B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.
Anti-ATP5B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-ATP5B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.
Anti-ATP5B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ATP5B mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-ATP5B mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.
Anti-ATP5B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP5B.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-ATP5B antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ATP5B (Red) or empty vector control plasmid (Blue) were immunostained with anti-ATP5B mouse monoclonal, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/24/2018

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