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Anti-ATG4D Antibody (aa220-249) LS-C156608

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ATG4D Antibodies

Anti-ATG4D Antibody (aa444-457) IHC-plus™ LS-B6488
Goat Polyclonal to Human ATG4D
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ATG4D Antibody (clone 222CT15.4.1, APC) LS-C221250
Mouse Monoclonal [clone 222CT15.4.1] (IgG1,k) to Human ATG4D
Human
ICC, Immunofluorescence, Western blot, ELISA
APC Conjugated
Anti-ATG4D Antibody (clone 222CT15.4.1, FITC) LS-C221253
Mouse Monoclonal [clone 222CT15.4.1] (IgG1,k) to Human ATG4D
Human
ICC, Immunofluorescence, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ATG4D
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human ATG4D
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)

Specificity and Use

ATG4D antibody was raised against kLH-conjugated synthetic peptide between amino acids 220-249 from internal region of human ATG4D.
LS-E5593 - Lyophilized - 100 µg - $145.00
aa220-249
Human AUTL4 / ATG4D

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ATG4D

Q86TL0 NM_032885 NP_116274.3

ATG4D Antibody, APG4-D Antibody, APG4D Antibody, AUTL4 Antibody, APG4 autophagy 4 homolog D Antibody, Cysteine protease ATG4D Antibody, Autophagin-4 Antibody

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot
APG4D Antibody (L235) western blot of HL-60 cell line lysates (35 ug/lane). The APG4D antibody detected the APG4D protein (arrow).

Western blot

Western blot
Western blot of anti-APG4D antibody in mouse kidney tissue lysate. APG4D(arrow) was detected using the purified antibody.

Western blot

Western blot
Western blot of anti-hAPG4D-L235 antibody in 293 cell line lysates transiently transfected with the APG4D gene (2 ug/lane). hAPG4D-L235(arrow) was detected using the purified antibody.

Requested From: United States
Date Requested: 12/9/2016

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