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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATG3 Antibody (aa83‑112) LS‑C156581
ATG3 antibody LS-C156581 is an unconjugated rabbit polyclonal antibody to human ATG3. Validated for WB.
Catalog
Size
Price
LS-C156581-400
400 µl
Unavailable

Popular ATG3 Products

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunofluorescence analysis of Hela cells using ATG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
Species: Human
Applications: IHC, ICC, Western blot, ELISA
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ATG3 mouse mAb with DAB staining.
Species: Human
Applications: IHC, ICC, Western blot, Flow Cytometry, ELISA

Product Description

ATG3 antibody LS-C156581 is an unconjugated rabbit polyclonal antibody to human ATG3. Validated for WB.
About ATG3
E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. Q9NT62 NM_022488 NP_071933.2

ATG3 Antibody, APG3-LIKE Antibody, APG3 Antibody, APG3L Antibody, Autophagy related 3 Antibody, HApg3 Antibody, PC3-96 Antibody, Protein PC3-96 Antibody, Autophagy-related protein 3 Antibody


Specifications

Target
Human ATG3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E5553 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156581. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa83-112
Specificity
This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-112 amino acids from the C-terminal region of human ATG3.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.
Western blot of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.

Western blot

APG3L Antibody (I98) western blot of HL-60 cell line lysates (35 ug/lane). The APG3L antibody detected the APG3L protein (arrow).
APG3L Antibody (I98) western blot of HL-60 cell line lysates (35 ug/lane). The APG3L antibody detected the APG3L protein (arrow).

Western blot

Western blot of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.
Western blot of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.

Western blot

APG3L Antibody (I98) western blot of HL-60 cell line lysates (35 ug/lane). The APG3L antibody detected the APG3L protein (arrow).
APG3L Antibody (I98) western blot of HL-60 cell line lysates (35 ug/lane). The APG3L antibody detected the APG3L protein (arrow).

Western blot

Western blot of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.
Western blot of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.

Western blot

APG3L Antibody (I98) western blot of HL-60 cell line lysates (35 ug/lane). The APG3L antibody detected the APG3L protein (arrow).
APG3L Antibody (I98) western blot of HL-60 cell line lysates (35 ug/lane). The APG3L antibody detected the APG3L protein (arrow).

Western blot

Western blot of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.
Western blot of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene.

Western blot

APG3L Antibody (I98) western blot of HL-60 cell line lysates (35 ug/lane). The APG3L antibody detected the APG3L protein (arrow).
APG3L Antibody (I98) western blot of HL-60 cell line lysates (35 ug/lane). The APG3L antibody detected the APG3L protein (arrow).


Requested From: 
Date Requested: 6/22/2018

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