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Anti-ASCL2 Antibody (aa1-49) LS-C149854


Wt. Vol. Conc. Price
100 µg - - Unavailable

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Sheep Polyclonal (IgG) to Human ASCL2
IHC - Paraffin, Western blot


Human ASCL2
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

ASCL2 antibody was raised against e. coli-derived recombinant human ASCL2/Mash2 Met1-Ala49 Accession # Q99929.
Detects human ASCL2/Mash2 in Western blots.


Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About ASCL2

Q99929 NM_005170 NP_005161.1

ASCL2 Antibody, Achaete-scute homolog 2 Antibody, Achaete-scute complex-like 2 Antibody, ASH-2 Antibody, ASH2 Antibody, BHLHa45 Antibody, HASH2 Antibody, MASH2 Antibody

ASCL2 is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.


ASCL2/Mash2 in Human Intestine. ASCL2/Mash2 was detected in immersion fixed paraffin-embedded sections of human intestine using Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to the base of intestinal crypts.

Western blot

Western blot
Detection of Human ASCL2/Mash2 by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 ug/ml of Sheep Anti-Human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for ASCL2/Mash2 at approximately 20 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: 
Date Requested: 4/23/2017

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