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Anti-ARID5B Antibody (aa500-550) IHC-plus™ LS-B7987

Note: This antibody replaces LS-C148609

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ARID5B Antibodies

Anti-ARID5B Antibody (aa500-550) IHC-plus™ LS-B7987
Rabbit Polyclonal to Human ARID5B
Human, Monkey, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ARID5B Antibody (aa389-418) LS-C159115
Rabbit Polyclonal to Human ARID5B
Human
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ARID5B
Human, Monkey, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human ARID5B
Rabbit
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (2 - 4 µg/ml)

Specificity and Use

ARID5B antibody was raised against a portion of amino acids 500-550 of MRF2 was used as the immunogen.
aa500-550
Human ARID5B

Packaging

PBS, 0.05% BSA, 0.05% sodium azide
Long term: -20°C; Short term: -20°C
For research use only.

About ARID5B

Q14865 M73837 AAA59870.1

ARID5B Antibody, DESRT Antibody, Modulator recognition factor 2 Antibody, MRF2 Antibody, MRF-2 Antibody, MRF1-like protein Antibody

Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver.

Immunohistochemistry

Immunohistochemistry
Anti-ARID5B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7987 dilution 10 ug/ml.

Western blot

Western blot
Western blot of MRF2 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using LS-B7987 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Western blot

Western blot
Western blot of MRF2 in human U87 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate using LS-B7987 at 3 ug/ml, 3 ug/ml and 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
 

Requested From: United States
Date Requested: 12/3/2016

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