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Anti-ARFGAP3 Antibody (N-Terminus) LS-C155439

Catalog Size Price
LS-C155439-100 100 µl (1 mg/ml) Unavailable

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24 ARFGAP3 Antibodies

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Anti-ARFGAP3 Antibody LS-C209178
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Immunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent signal.
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Anti-ARFGAP3 Antibody LS-C660054
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100% Guaranteed
Rabbit Polyclonal (IgG) to Human ARFGAP3
Western blot


Human (tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


Western blot (1:500 - 1:3000)

Specificity and Use

ARFGAP3 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 170 of ARFGAP3 (SwissProt Q9NP61). Percent identity by BLAST analysis: Human (100%); Mouse (91%); Xenopus (83%).
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.


PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
For research use only.


Q9NP61 NM_014570 NP_055385.3

ARFGAP3 Antibody, ARF GAP 3 Antibody

ARFGAP3 is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids.

Western blot

Sample (30 ug of whole cell lysate) A: A549 B: HeLa 7.5% SDS PAGE ARFGAP3 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 B: HeLa 7.5% SDS PAGE ARFGAP3 antibody diluted at 1:1000

Requested From: 
Date Requested: 4/22/2018

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