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Anti-ARF6 Antibody (aa126-155) LS-C156452

Catalog Size Price
LS-C156452-50 50 µg Unavailable

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65 ARF6 Antibodies

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100% Guaranteed
Rabbit Polyclonal to Human ARF6
Human
IHC, Western blot, Flow Cytometry
Unconjugated

Details

Human ARF6
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

ARF6 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human ARF6.
aa126-155
Human ARF6

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ARF6

P62330 NM_001663 NP_001654.1

ARF6 Antibody, ADP-ribosylation factor 6 Antibody

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARF6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot

Western blot
Western blot analysis of ARF6 (arrow) using rabbit polyclonal ARF6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARF6 gene (Lane 2).

Western blot

Western blot
Western blot analysis of ARF6 Antibody (C-term) in T47D,MCF-7 cell line lysates (35 ug/lane). ARF6 (arrow) was detected using the purified Pab.(2 ug/ml)

Flow Cytometry

Flow Cytometry
ARF6 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 6/26/2017

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