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Anti-AREG / Amphiregulin Antibody LS-C150151

Catalog Size Price
LS-C150151-25 25 µg (1 mg/ml) Unavailable
LS-C150151-100 100 µg (0.2 mg/ml) Unavailable

Most Popular AREG / Amphiregulin Antibodies

Anti-AREG / Amphiregulin Antibody LS-C88721
Rabbit Polyclonal (IgG) to Human AREG / Amphiregulin
Human, Mouse
Immunoprecipitation
Unconjugated
Anti-AREG / Amphiregulin Antibody (aa91-140) LS-C134906
Rabbit Polyclonal to Human AREG / Amphiregulin
Human
Western blot
Unconjugated
Western blot Image
Anti-AREG / Amphiregulin Antibody LS-C149881
Goat Polyclonal (IgG) to Human AREG / Amphiregulin
Human
IHC, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AREG / Amphiregulin Antibody LS-C150151
Goat Polyclonal (IgG) to Mouse AREG / Amphiregulin
Mouse
IHC - Frozen, Western blot, ELISA, Neutralization
Unconjugated
Neutralization Image
Anti-AREG / Amphiregulin Antibody LS-C341363
Rabbit Polyclonal to Human AREG / Amphiregulin
Human
IHC, Western blot
Unconjugated

100% Guaranteed
Goat Polyclonal (IgG) to Mouse AREG / Amphiregulin
Mouse
IHC - Frozen, Western blot, ELISA, Neutralization
Unconjugated

Details

Mouse AREG / Amphiregulin
Goat
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Frozen (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • ELISA
  • Neutralization

Specificity and Use

E. coli-derived recombinant mouse Amphiregulin Ser94-Lys 191 Accession # P31955.
Detects mouse Amphiregulin in ELISAs. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Amphiregulin is.
Western Blot, 0.1 µg/ml, Recombinant Mouse Amphiregulin. Immunohistochemistry, 5-15 µg/ml, Perfusion fixed frozen sections of rat intestine and skin. Mouse Amphiregulin Sandwich Immunoassay, Reagent. ELISA Capture, 0.2-0.8 µg/ml, Mouse Amphiregulin Antibody. ELISA Detection, 0.1-0.4 µg/ml, Mouse Amphiregulin Biotinylated Antibody. Standard, Recombinant Mouse Amphiregulin. Neutralization, Measured by its ability to neutralize Amphiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast. cell line. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/ml in the presence of 50 ng/ml Recombinant Mouse Amphiregulin.Endotoxin level: <0.10 EU per 1 µg of the antibody by the LAL method.

Packaging

0.2 um filtered solution in PBS.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About AREG / Amphiregulin

P15514 NM_001657 NP_001648.1

AREG Antibody, Amphiregulin Antibody, CRDGF Antibody, SDGF Antibody

AREG / Amphiregulin is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines.

Neutralization

Neutralization
Cell Proliferation Induced by Amphiregulin and Neutralization by Mouse Amphiregulin Antibody. Recombinant Mouse Amphiregulin stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Amphiregulin (50 ng/ml) is neutralized (green line) by increasing concentrations of Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.3-1.5 ug/ml.

Requested From: 
Date Requested: 8/19/2017

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