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Anti-APRT Antibody (aa12-40) LS-C156439

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular APRT Antibodies

Anti-APRT Antibody (aa143-170) IHC-plus™ LS-B9158
Rabbit Polyclonal to Human APRT
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-APRT Antibody (N-Terminus) IHC-plus™ LS-B10419
Rabbit Polyclonal (IgG) to Human APRT
Human, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-APRT Antibody (aa1-180, FITC) LS-C303208
Rabbit Polyclonal (IgG) to Human APRT
Human
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human APRT
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human APRT
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

APRT antibody was raised against kLH-conjugated synthetic peptide between amino acids 12-40 from N-terminal region of human APRT.
LS-E7184 - Lyophilized - 100 µg - $145.00
aa12-40
Human APRT

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About APRT

P07741 NM_000485 NP_000476.1

APRT Antibody, AMP Antibody, AMP diphosphorylase Antibody, APRTD Antibody, AMP pyrophosphorylase Antibody, Transphosphoribosidase Antibody

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent image of A549 cell stained with APRT Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Western blot

Western blot
APRT Antibody western blot of A2058 cell line lysates (35 ug/lane). The APRT antibody detected the APRT protein (arrow).

Flow Cytometry

Flow Cytometry
Flow cytometric of HepG2 cells using APRT Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/2/2016

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