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Anti-APP / Beta Amyloid Precursor Antibody (aa18-289, clone J4H9) IHC-plus™ LS-B2970

Note: This antibody replaces LS-C93706

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Mouse Monoclonal [clone J4H9] (IgG2b,k) to Human APP / Beta Amyloid Precursor
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human APP / Beta Amyloid Precursor
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [J4H9]
Protein G purified

Applications

IHC - Paraffin (2.5 µg/ml), Western blot (1:500 - 1:1000), ELISA

Specificity and Use

APP / Beta Amyloid Precursor antibody was raised against recombinant human APP (18-289aa) purified from E. coli.
aa18-289
Immunohistochemistry: LS-B2970 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2970 was determined to be 2.5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About APP / Beta Amyloid Precursor

P05067 NM_000484 NP_000475.1

APP Antibody, ABPP Antibody, A4 Antibody, ABETA Antibody, AD1 Antibody, Alzheimer disease Antibody, Amyloid beta Antibody, Beta-amyloid peptide Antibody, APPI Antibody, Beta amyloid Antibody, CVAP Antibody, AAA Antibody, PN2 Antibody, PreA4 Antibody, Amyloid beta A4 protein Antibody, Peptidase nexin-II Antibody, CTFgamma Antibody, PN-II Antibody, Protease nexin-II Antibody


Immunohistochemistry

Immunohistochemistry
Anti-APP antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2970 concentration 5 ug/ml.

Western blot

Western blot
 

Requested From: United States
Date Requested: 8/4/2015

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