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Anti-APOB / Apolipoprotein B Antibody (aa3984-3995) IHC-plus™ LS-B3022

Note: This antibody replaces LS-C87337

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular APOB / Apolipoprotein B Antibodies

Anti-APOB / Apolipoprotein B Antibody IHC-plus™ LS-B3646
Goat Polyclonal to Human APOB / Apolipoprotein B
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-APOB / Apolipoprotein B Antibody IHC-plus™ LS-B6180
Rabbit Polyclonal (IgG) to Mouse APOB / Apolipoprotein B
Mouse, Human
IHC - Paraffin, Western blot, Immunodiffusion
Unconjugated
Immunohistochemistry Image
Anti-APOB / Apolipoprotein B Antibody LS-C149851
Mouse Monoclonal (IgG2b) to Human APOB / Apolipoprotein B
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human APOB / Apolipoprotein B
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human APOB / Apolipoprotein B
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:32000)

Specificity and Use

APOB / Apolipoprotein B antibody was raised against synthetic peptide C-TDLHLRYQKDKK from an internal region of human APOB (NP_000375.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%).
LS-E26813 - Lyophilized - 100 µg - $145.00
aa3984-3995
Human APOB.
Immunohistochemistry: LS-B3022 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3022 was determined to be 2 ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Band corresponding to the expected size of ApoB100 is seen along with additional bands at lower molecular weights.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About APOB / Apolipoprotein B

P04114 NM_000384 NP_000375.2

APOB Antibody, ApoB-100 Antibody, Apolipoprotein B-48 Antibody, ApoB-48 Antibody, Apolipoprotein B Antibody, FLDB Antibody, LDLCQ4 Antibody, Mutant Apo B 100 Antibody, Apo B-100 Antibody, Apolipoprotein B-100 Antibody, Apolipoprotein B48 Antibody

Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16 kb. The 2 proteins share a common amino terminal sequence.

Immunohistochemistry

Immunohistochemistry
Anti-Apolipoprotein B antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3022 concentration 2 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3022 concentration 2 ug/ml.

Requested From: United States
Date Requested: 12/2/2016

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