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Anti-APOA4 Antibody (aa359-371) IHC-plus™ LS-B2417

Note: This antibody replaces LS-C54517, LS-C69164, LS-B2840

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular APOA4 Antibodies

Anti-APOA4 Antibody (aa359-371) IHC-plus™ LS-B2417
Goat Polyclonal to Human APOA4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-APOA4 Antibody (aa93-108) LS-C87355
Goat Polyclonal to Mouse APOA4
Mouse
ELISA
Unconjugated
Anti-APOA4 Antibody (aa21-396, clone 2D1C9) IHC-plus™ LS-B3885
Mouse Monoclonal [clone 2D1C9] (IgG1) to Human APOA4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Goat Polyclonal to Human APOA4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human APOA4
Goat
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • ELISA (1:32000)

Specificity and Use

APOA4 antibody was raised against synthetic peptide C-KEKESQDKTLSLP from an internal region (near the C-terminus) of human APOA4 (NP_000473.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Baboon, Monkey, Galago (92%); Marmoset, Armadillo (85%).
LS-E26242 - Lyophilized - 100 µg - $145.00
aa359-371
Human APOA4.
Immunohistochemistry: LS-B2417 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2417 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (1)

1
Identification of potential bladder cancer markers in urine by abundant-protein depletion coupled with quantitative proteomics. Chen CL, Lin TS, Tsai CH, Wu CC, Chung T, Chien KY, Wu M, Chang YS, Yu JS, Chen YT. Journal of proteomics. 2013 Jun;85:28-43. [Full Text Article] [PubMed:23631828]

About APOA4

P06727 NM_000482 NP_000473.2

APOA4 Antibody, Apo-AIV Antibody, Apolipoprotein A-IV Antibody, Apolipoprotein A4 Antibody, ApoA-IV Antibody

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

Immunohistochemistry

Immunohistochemistry
Anti-APOA4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2417 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.01 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/4/2016

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