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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AP50 / AP2M1 Antibody (aa1‑150) LS‑C185408
AP2M1 antibody LS-C185408 is an unconjugated rabbit polyclonal antibody to AP2M1 (AP50) from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C185408-100
100 µl (0.57 mg/ml)
Unavailable

Popular AP50 / AP2M1 Products

Sample (30 ug of whole cell lysate). A: Hep G2. 10% SDS PAGE. AP2M1 antibody diluted at 1:1000.
Species: Human
Applications: Western blot
IHC of paraffin-embedded Human Kidney tissue using anti-AP2M1 mouse monoclonal antibody.
Species: Human, Rat, Dog
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded rat kidney using AP2M1 antibody at dilution of 1:100 (200x lens).
Species: Human, Mouse, Rat
Applications: IHC, Western blot
IHC of paraffin-embedded Human colon tissue using anti-AP2M1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

AP2M1 antibody LS-C185408 is an unconjugated rabbit polyclonal antibody to AP2M1 (AP50) from human, mouse, rat and other species. Validated for IHC and WB.
About AP50 / AP2M1
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. Q96CW1 NM_004068 NP_004059.2

AP2M1 Antibody, Adaptin-mu2 Antibody, AP-2 complex subunit mu Antibody, AP-2 mu 2 chain Antibody, AP-2 mu chain Antibody, AP50 Antibody, CLAPM1 Antibody, Mu2 Antibody, KIAA0109 Antibody, HA2 50 kDa subunit Antibody


Specifications

Target
Human AP50 / AP2M1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Predicted
Pig, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AP50 / AP2M1 antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 150 (Q96CW1) of AP2M1. Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine (100%); Pig, Xenopus (99%); Chicken (97%).
Epitope
aa1-150
Specificity
Human AP50 / AP2M1
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using AP2M1 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using AP2M1 antibody at 1:100 dilution.

Western blot

Sample (30 ug of whole cell lysate). A:293T, B: HeLa S3. 10% SDS PAGE. AP50 / AP2M1 antibody diluted at 1:500
Sample (30 ug of whole cell lysate). A:293T, B: HeLa S3. 10% SDS PAGE. AP50 / AP2M1 antibody diluted at 1:500

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using AP2M1 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using AP2M1 antibody at 1:100 dilution.

Western blot

Sample (30 ug of whole cell lysate). A:293T, B: HeLa S3. 10% SDS PAGE. AP50 / AP2M1 antibody diluted at 1:500
Sample (30 ug of whole cell lysate). A:293T, B: HeLa S3. 10% SDS PAGE. AP50 / AP2M1 antibody diluted at 1:500

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using AP2M1 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using AP2M1 antibody at 1:100 dilution.

Western blot

Sample (30 ug of whole cell lysate). A:293T, B: HeLa S3. 10% SDS PAGE. AP50 / AP2M1 antibody diluted at 1:500
Sample (30 ug of whole cell lysate). A:293T, B: HeLa S3. 10% SDS PAGE. AP50 / AP2M1 antibody diluted at 1:500

Immunohistochemistry

IHC of paraffin-embedded N87 xenograft using AP2M1 antibody at 1:100 dilution.
IHC of paraffin-embedded N87 xenograft using AP2M1 antibody at 1:100 dilution.

Western blot

Sample (30 ug of whole cell lysate). A:293T, B: HeLa S3. 10% SDS PAGE. AP50 / AP2M1 antibody diluted at 1:500
Sample (30 ug of whole cell lysate). A:293T, B: HeLa S3. 10% SDS PAGE. AP50 / AP2M1 antibody diluted at 1:500


Requested From: 
Date Requested: 6/24/2018

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