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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ANXA11 / Annexin XI Antibody (clone 1C6) LS‑C115005
Annexin XI antibody LS-C115005 is an unconjugated mouse monoclonal antibody to human Annexin XI (ANXA11). Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C115005-100
100 µl
Unavailable

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Product Description

Annexin XI antibody LS-C115005 is an unconjugated mouse monoclonal antibody to human Annexin XI (ANXA11). Validated for Flow, IF and WB.
About ANXA11 / Annexin XI
ANXA11 / Annexin XI is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. P50995 NM_001157 NP_001148.1

ANXA11 Antibody, 56 kDa autoantigen Antibody, Annexin A11 Antibody, Annexin XI Antibody, CAP-50 Antibody, Annexin-11 Antibody, ANX11 Antibody, Autoantigen, 56-kD Antibody, CAP50 Antibody


Specifications

Target
Human ANXA11 / Annexin XI
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1C6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
Full length human recombinant protein of human ANXA11 (NP_001148) produced in HEK293T cell.
Specificity
Human Annexin XI / Annexin A11
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunofluorescence

Anti-ANXA11 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11.
Anti-ANXA11 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-ANXA11 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11.
Anti-ANXA11 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-ANXA11 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11.
Anti-ANXA11 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal, and then analyzed by flow cytometry.

Immunofluorescence

Anti-ANXA11 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11.
Anti-ANXA11 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/21/2018

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