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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ANAPC1 / APC1 Antibody (C‑Terminus) IHC‑plus™ LS‑B6979
Note: This antibody replaces LS-C108705
APC1 antibody LS-B6979 is an unconjugated rabbit polyclonal antibody to APC1 (ANAPC1) from human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6979-50
50 µg (1 mg/ml)
Unavailable

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Product Description

APC1 antibody LS-B6979 is an unconjugated rabbit polyclonal antibody to APC1 (ANAPC1) from human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ANAPC1 / APC1
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Q9H1A4 NM_022662 NP_073153.1

ANAPC1 Antibody, APC1 Antibody, Cyclosome subunit 1 Antibody, MCPR Antibody, Mitotic checkpoint regulator Antibody, TSG24 Antibody


Specifications

Target
Human ANAPC1 / APC1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30910 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B6979. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
APC1 antibody can be used for detection of APC1 by Western blot at 1-2 ug/ml.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ANAPC1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6979 concentration 5 ug/ml.
Anti-ANAPC1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6979 concentration 5 ug/ml.

Western blot

Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-ANAPC1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6979 concentration 5 ug/ml.
Anti-ANAPC1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6979 concentration 5 ug/ml.

Western blot

Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-ANAPC1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6979 concentration 5 ug/ml.
Anti-ANAPC1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6979 concentration 5 ug/ml.

Western blot

Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry

Anti-ANAPC1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6979 concentration 5 ug/ml.
Anti-ANAPC1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6979 concentration 5 ug/ml.

Western blot

Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.
Western blot of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.


Requested From: 
Date Requested: 6/22/2018

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