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Anti-AML1 / RUNX1 Antibody (Ser276) LS-C162813

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AML1 / RUNX1 Antibodies

Anti-AML1 / RUNX1 Antibody (N-Terminus) IHC-plus™ LS-B998
Rabbit Polyclonal to Human AML1 / RUNX1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AML1 / RUNX1 Antibody (clone 4E7) IHC-plus™ LS-B5383
Mouse Monoclonal [clone 4E7] (IgG2b,k) to Human AML1 / RUNX1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AML1 / RUNX1 Antibody (Internal, clone 15B) LS-C191744
Rabbit Polyclonal [clone 15B] (IgG) to Human AML1 / RUNX1
Human, Mouse
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human AML1 / RUNX1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human AML1 / RUNX1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

AML1 / RUNX1 antibody was raised against kLH-conjugated synthetic peptide between amino acids 227-255 from human RUNX1.
LS-E7114 - Lyophilized - 100 µg - $145.00
Ser276
Human AML1 / RUNX1

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About AML1 / RUNX1

Q01196 NM_001754 NP_001745.2

RUNX1 Antibody, AMLCR1 Antibody, Aml1 oncogene Antibody, AML1 Antibody, AML1-EVI-1 Antibody, CBF-alpha-2 Antibody, CBFA2 Antibody, Acute myeloid leukemia 1 Antibody, EVI-1 Antibody, PEBP2A2 Antibody, PEA2-alpha B Antibody, AML1-EVI-1 fusion protein Antibody, Oncogene AML-1 Antibody, PEBP2-alpha B Antibody, PEBP2aB Antibody

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Western blot

Western blot
RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).

Flow Cytometry

Flow Cytometry
RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/9/2016

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