LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
AML1 / RUNX1 Antibody (Ser276) LS‑C162813
RUNX1 antibody LS-C162813 is an unconjugated rabbit polyclonal antibody to human RUNX1 (AML1). Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C162813-400
400 µl
Unavailable

Popular AML1 / RUNX1 Products

Anti-RUNX1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B998 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Anti-RUNX1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5382 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-RUNX1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5383 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

RUNX1 antibody LS-C162813 is an unconjugated rabbit polyclonal antibody to human RUNX1 (AML1). Validated for Flow, IF, IHC and WB.
About AML1 / RUNX1
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Q01196 NM_001754 NP_001745.2

RUNX1 Antibody, AMLCR1 Antibody, Aml1 oncogene Antibody, AML1 Antibody, AML1-EVI-1 Antibody, CBF-alpha-2 Antibody, CBFA2 Antibody, Acute myeloid leukemia 1 Antibody, EVI-1 Antibody, PEBP2A2 Antibody, PEA2-alpha B Antibody, AML1-EVI-1 fusion protein Antibody, Oncogene AML-1 Antibody, PEBP2-alpha B Antibody, PEBP2aB Antibody


Specifications

Target
Human AML1 / RUNX1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E7114 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C162813. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Ser276
Specificity
This RUNX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from human RUNX1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Western blot

RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).
RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).

Flow Cytometry

RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Western blot

RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).
RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).

Flow Cytometry

RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Western blot

RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).
RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).

Flow Cytometry

RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Confocal immunofluorescence of RUNX1 Antibody (S276) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Western blot

RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).
RUNX1 Antibody (S276) western blot of U-937 cell line lysates (35 ug/lane). The RUNX1 antibody detected the RUNX1 protein (arrow).

Flow Cytometry

RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
RUNX1 Antibody (S276) flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/19/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number