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AML1 / RUNX1 Antibody (clone 4E7) IHC‑plus™ LS‑B5383
Note: This antibody replaces LS-C133012
RUNX1 antibody LS-B5383 is an unconjugated mouse monoclonal antibody to RUNX1 (AML1) from human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
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Review This ProductPopular AML1 / RUNX1 Products
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Product Description
RUNX1 antibody LS-B5383 is an unconjugated mouse monoclonal antibody to RUNX1 (AML1) from human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About AML1 / RUNX1
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter.
Q01196
NM_001754
NP_001745.2
RUNX1 Antibody, AMLCR1 Antibody, Aml1 oncogene Antibody, AML1 Antibody, AML1-EVI-1 Antibody, CBF-alpha-2 Antibody, CBFA2 Antibody, Acute myeloid leukemia 1 Antibody, EVI-1 Antibody, PEBP2A2 Antibody, PEA2-alpha B Antibody, AML1-EVI-1 fusion protein Antibody, Oncogene AML-1 Antibody, PEBP2-alpha B Antibody, PEBP2aB Antibody
Specifications
Target
Human AML1 / RUNX1
Host
Mouse
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
[4E7]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (10 µg/ml)
- Western blot
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
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Immunogen
AML1 / RUNX1 antibody was raised against rUNX1 (NP_001001890, 210 a.a. ~ 311 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human RUNX1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Immunohistochemistry
Anti-RUNX1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5383 concentration 5 ug/ml.
Immunofluorescence
![Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com/image2/aml1-runx1-antibody-clone-4e7-ihc-plus-ls-b5383/50065_1226893.jpg)
Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7 Western blot of RUNX1 expression in HeLa NE.
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7. Western blot of RUNX1 expression in NIH/3T3.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RUNX1 is approximately 1 ng/ml as a capture antibody.
Immunohistochemistry
Anti-RUNX1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5383 concentration 5 ug/ml.
Immunofluorescence
![Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com/image2/aml1-runx1-antibody-clone-4e7-ihc-plus-ls-b5383/50065_3005292.jpg)
Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7 Western blot of RUNX1 expression in HeLa NE.
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7. Western blot of RUNX1 expression in NIH/3T3.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RUNX1 is approximately 1 ng/ml as a capture antibody.
Immunohistochemistry
Anti-RUNX1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5383 concentration 5 ug/ml.
Immunofluorescence
![Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com/image2/aml1-runx1-antibody-clone-4e7-ihc-plus-ls-b5383/50065_129641.jpg)
Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7 Western blot of RUNX1 expression in HeLa NE.
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7. Western blot of RUNX1 expression in NIH/3T3.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RUNX1 is approximately 1 ng/ml as a capture antibody.
Immunohistochemistry
Anti-RUNX1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5383 concentration 5 ug/ml.
Immunofluorescence
Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7 Western blot of RUNX1 expression in HeLa NE.
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7. Western blot of RUNX1 expression in NIH/3T3.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RUNX1 is approximately 1 ng/ml as a capture antibody.
Requested From:
Date Requested: 6/23/2018
Date Requested: 6/23/2018
