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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
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IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Mouse Monoclonal [clone 4E7] (IgG2b,k) to Human AML1 / RUNX1
Human, Mouse
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Details
Human AML1 / RUNX1
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [4E7]
Unconjugated
Purified from ascites by Protein A
Unmodified
Applications
IHC
IHC - Paraffin (5 µg/ml)
Immunofluorescence (10 µg/ml)
Western blot
ELISA
Specificity and Use
AML1 / RUNX1 antibody was raised against rUNX1 (NP_001001890, 210 a.a. ~ 311 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter.
Anti-RUNX1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5383 concentration 5 ug/ml.
Immunofluorescence
Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7 Western blot of RUNX1 expression in HeLa NE.
Western blot
RUNX1 monoclonal antibody (M05), clone 4E7. Western blot of RUNX1 expression in NIH/3T3.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RUNX1 is approximately 1 ng/ml as a capture antibody.