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Anti-AML1 / RUNX1 Antibody (clone 4E7) IHC-plus™ LS-B5383

Note: This antibody replaces LS-C133012

Ordering

Wt. Vol. Conc. Price
50 µg - 0.46 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AML1 / RUNX1 Antibodies

Anti-AML1 / RUNX1 Antibody (N-Terminus) IHC-plus™ LS-B998
Rabbit Polyclonal to Human AML1 / RUNX1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AML1 / RUNX1 Antibody (clone 4E7) IHC-plus™ LS-B5383
Mouse Monoclonal [clone 4E7] (IgG2b,k) to Human AML1 / RUNX1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AML1 / RUNX1 Antibody (Internal, clone 15B) LS-C191744
Rabbit Polyclonal [clone 15B] (IgG) to Human AML1 / RUNX1
Human, Mouse
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4E7] (IgG2b,k) to Human AML1 / RUNX1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human AML1 / RUNX1
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [4E7]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

AML1 / RUNX1 antibody was raised against rUNX1 (NP_001001890, 210 a.a. ~ 311 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human RUNX1
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Recombinant protein.

Packaging

PBS, pH 7.4.
For long term, store at -20°C. Avoid repeat freeze-thaw cycles.
For research use only.

About AML1 / RUNX1

Q01196 NM_001754 NP_001745.2

RUNX1 Antibody, AMLCR1 Antibody, Aml1 oncogene Antibody, AML1 Antibody, AML1-EVI-1 Antibody, CBF-alpha-2 Antibody, CBFA2 Antibody, Acute myeloid leukemia 1 Antibody, EVI-1 Antibody, PEBP2A2 Antibody, PEA2-alpha B Antibody, AML1-EVI-1 fusion protein Antibody, Oncogene AML-1 Antibody, PEBP2-alpha B Antibody, PEBP2aB Antibody

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter.

Immunohistochemistry

Immunohistochemistry
Anti-RUNX1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5383 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
RUNX1 monoclonal antibody (M05), clone 4E7 Western blot of RUNX1 expression in HeLa NE.

Western blot

Western blot
RUNX1 monoclonal antibody (M05), clone 4E7. Western blot of RUNX1 expression in NIH/3T3.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged RUNX1 is approximately 1 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/11/2016

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