Bovine ALPI / Alkaline Phosphatase

Sheep

Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Lyophilized

Alkaline Phosphatase [Calf intestine].

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.

• Western blot (1:250)
• ELISA (1:4000 - 1:20000)

Anti-Alkaline phosphatase has been assayed against 1.0 ug of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09923 NCBI: NM_001631 NP_001622.2