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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Alpha4GnT / A4GNT Antibody (aa251‑300) LS‑C81007
A4GNT antibody LS-C81007 is an unconjugated rabbit polyclonal antibody to A4GNT (Alpha4GnT) from human, rat and horse. Validated for WB.
Catalog
Size
Price
LS-C81007-50
50 µg
$365

Popular Alpha4GnT / A4GNT Products

Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemistry Image
Species: Human
Applications: IHC, Western blot, ELISA
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-A4GNT mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemical analysis of A4GNT staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of Human lung cancer using A4GNT Polyclonal Antibody at dilution of 1:30.
Species: Human
Applications: IHC, ELISA

Product Description

A4GNT antibody LS-C81007 is an unconjugated rabbit polyclonal antibody to A4GNT (Alpha4GnT) from human, rat and horse. Validated for WB.
About Alpha4GnT / A4GNT
Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. Q9UNA3 NM_016161 NP_057245.1

A4GNT Antibody, Alpha4GnT Antibody


Specifications

Target
Human Alpha4GnT / A4GNT
Host
Rabbit
Reactivity
Human, Rat, Horse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa251-300 of human A4GNT (Q9UNA3, NP_057245). Percent identity by BLAST analysis: Human, Opossum (100%); Gorilla, Gibbon, Monkey, Galago, Marmoset, Dog, Bovine, Bat, Rabbit (92%); Mouse, Guinea pig (91%); Turkey (90%); Rat (85%); Zebra finch, Chicken, Platypus, Lizard (83%).
Blocking Peptide
LS-E17124 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81007. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa251-300
Specificity
Human A4GNT
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/25/2018

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