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Antibodies > AKT2 > LS-C98942
 
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Catalog Number Size Price
LS-C98942-400 400 µl $393 
AKT2 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AKT2 Antibody - Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (S474). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AKT2 immunoreactivity is localized to Cytoplasm significantly.
AKT2 Antibody - Western blot of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
AKT2 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AKT2 Antibody - Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (S474). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AKT2 immunoreactivity is localized to Cytoplasm significantly.
AKT2 Antibody - Western blot of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
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Polyclonal Rabbit anti‑Human AKT2 Antibody (Ser474, IHC, IF, WB) LS‑C98942

Polyclonal Rabbit anti‑Human AKT2 Antibody (Ser474, IHC, IF, WB) LS‑C98942

Submit Review LSBio Guarantee
Antibody:
AKT2 Rabbit anti-Human Polyclonal (Ser474) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98942-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT2 Rabbit anti-Human Polyclonal (Ser474) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AKT2 antibody LS-C98942 is an unconjugated rabbit polyclonal antibody to human AKT2 (Ser474). Validated for IF, IHC and WB.
Target
Human AKT2
Synonyms
AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
Ser474
Specificity
This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from human AKT2.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT2
P31751 NM_001626 NP_001617.1

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Featured Products

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AKT2 Antibody - IHC of paraffin-embedded liver tissue using anti-AKT2 mouse monoclonal antibody. (Dilution 1:50).
AKT2 Antibody (clone OTI4H7) LS‑C114889
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Product
AKT2 Antibody (aa214‑451, clone 148CT5.4.1.4, PE) LS‑C221216
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, ELISA
AKT2 Antibody - AKT2 Monoclonal Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
AKT2 Antibody (aa214‑451) LS‑C156232
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
AKT2 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AKT2 Antibody (aa416‑444) LS‑C98940
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
AKT2 ELISA Kit
Human AKT2 (Sandwich ELISA) ELISA Kit - LS-F4927
Reactivity: Human
Range: 0.313-20 ng/ml
See all AKT2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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