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Anti-AKT2 Antibody (Ser474) LS-C98942

Catalog Size Price
LS-C98942-400 400 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human AKT2
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human AKT2
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E8622 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98942. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Ser474
This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from human AKT2.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About AKT2

P31751 NM_001626 NP_001617.1

AKT2 Antibody, PKB beta Antibody, PKBBETA Antibody, PRKBB Antibody, Protein kinase Akt-2 Antibody, Protein kinase B beta Antibody, RAC-PK-beta Antibody, Rac protein kinase beta Antibody, RAC-BETA Antibody, HIHGHH Antibody, PKBB Antibody

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (S474). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AKT2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

Western blot
Western blot of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).

Requested From: 
Date Requested: 10/22/2017

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