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Anti-AKT2 Antibody (aa455-468) LS-C19061

Note: This antibody replaces LS-C3609

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AKT2 Antibodies

Anti-AKT2 Antibody (C-Terminus) IHC-plus™ LS-B1041
Rabbit Polyclonal to Human AKT2
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-AKT2 Antibody (clone 4H7) LS-C114889
Mouse Monoclonal [clone 4H7] (IgG1) to Human AKT2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-AKT2 Antibody (clone 148CT5.4.1.4, PE) LS-C221216
Mouse Monoclonal [clone 148CT5.4.1.4] (IgG1) to Human AKT2
Human, Mouse
IHC, ICC, Immunofluorescence, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human AKT2
Human
Western blot, ELISA
Unconjugated

Details

Human AKT2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:4000 - 1:16000)
  • ELISA

Specificity and Use

AKT2 antibody was raised against synthetic peptide from human Akt2.
aa455-468
aa 455-468 (of 481) of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
This antibody was tested by ELISA and immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1000 dilution showing a band at approximately 60 kD. A working dilution of 1:4000 to 1:16000 is suggested for this product in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide. Researchers should determine optimal titers for other applications.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below.
For research use only.

Publications (1)

1
Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase. Subramanyam M, Takahashi N, Hasegawa Y, Mohri T, Okada Y. The Journal of biological chemistry. 2010 285:6109-17. [PubMed:20048146] [PMC:PMC2825405]

About AKT2

P31751 NM_001626 NP_001617.1

AKT2 Antibody, PKB beta Antibody, PKBBETA Antibody, PRKBB Antibody, Protein kinase Akt-2 Antibody, Protein kinase B beta Antibody, RAC-PK-beta Antibody, Rac protein kinase beta Antibody, RAC-BETA Antibody, HIHGHH Antibody, PKBB Antibody

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Western blot

Western blot
Anti-AKT2 Antibody - Western Blot. Affinity Purified antibody to AKT2 was used at a 1:1000 dilution to detect AKT2 by Western blot of lysates from mouse tissues. The antibody is shown to react with mouse Akt2 in mouse liver, skeletal muscle and fat using standard western blotting methods.

Western blot

Western blot
Anti-AKT2 Antibody - Western Blot. Antibody to AKT2 detects both unphosphorylated and phosphorylated AKT2 in Western blot. As before approximately 20 mg/lane of crude HEK293 lysate was loaded for SDS-PAGE, separated and then transferred to nitrocellulose. The right lane contains lysate pretreated for 15min with 25 uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This antibody clearly detects both the phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2.

Western blot

Western blot
Anti-AKT2 Antibody - Western Blot. Anti-AKT2 Antibody in Western blot shows detection of AKT2. A lysate from a stable HEK293 cell line expressing an inducible, myristoylated form of Akt2 (MyrAkt2-ER) was loaded for SDS- PAGE, separated and then transferred to nitrocellulose. The blot was used at 1:1000 and reacted with Anti-AKT2 Antibody for 1h at room temperature. In response to 4OHT (tamoxifen), the Akt2 is recruited to the plasma membrane via its myristoylation sequence, and becomes phosphorylated and activated. The blot (right panel) shows 0 m, 15 m, and 30 m of 4OHT treatment. This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2. Endogenous Akt2 runs at about 60kD whereas the myristoylated construct runs at around 110kD. Antibody to AKT2 detects both unphosphorylated and phosphorylated AKT2. As before approximately 20 mg/lane of crude HEK293 lysate was loaded for SDS-PAGE, separated and then transferred to nitrocellulose. The right lane contains lysate pretreated-for-15min-with 25 uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This antibody clearly detects both the phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2.
 

Requested From: United States
Date Requested: 12/8/2016

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