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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT1S1 / PRAS40 Antibody (phospho‑Thr246) LS‑C53893
PRAS40 antibody LS-C53893 is an unconjugated rabbit polyclonal antibody to PRAS40 (AKT1S1) from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C53893-100
100 µg
Unavailable

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Product Description

PRAS40 antibody LS-C53893 is an unconjugated rabbit polyclonal antibody to PRAS40 (AKT1S1) from human and mouse. Validated for WB.
About AKT1S1 / PRAS40
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Q96B36 NM_032375 NP_115751.3

AKT1S1 Antibody, PRAS40 Antibody, Proline-rich AKT1 substrate 1 Antibody, Lobe Antibody


Specifications

Target
Human AKT1S1 / PRAS40
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Immunogen
AKT1S1 / PRAS40 antibody was raised against synthetic phosphopeptide derived from the region of human PRAS40 that contains threonine 246.
Epitope
pThr246
Specificity
Human and mouse (100% homologous) PRAS40
Presentation
Dulbecco's PBS (without Mg2+, Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, 1.0 mg/mL BSA (IgG, protease free) as a carrier.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Western blot

Extracts prepared from NIH3T3 cells left unstimulated (1) or stimulated with PDGF (2-6), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (delta) phosphatase (6) or untreated (1-5), w
Extracts prepared from NIH3T3 cells left unstimulated (1) or stimulated with PDGF (2-6), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (delta) phosphatase (6) or untreated (1-5), w

Western blot

Extracts prepared from NIH3T3 cells left unstimulated (1) or stimulated with PDGF (2-6), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (delta) phosphatase (6) or untreated (1-5), w
Extracts prepared from NIH3T3 cells left unstimulated (1) or stimulated with PDGF (2-6), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (delta) phosphatase (6) or untreated (1-5), w

Western blot

Extracts prepared from NIH3T3 cells left unstimulated (1) or stimulated with PDGF (2-6), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (delta) phosphatase (6) or untreated (1-5), w
Extracts prepared from NIH3T3 cells left unstimulated (1) or stimulated with PDGF (2-6), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (delta) phosphatase (6) or untreated (1-5), w

Western blot

Extracts prepared from NIH3T3 cells left unstimulated (1) or stimulated with PDGF (2-6), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (delta) phosphatase (6) or untreated (1-5), w
Extracts prepared from NIH3T3 cells left unstimulated (1) or stimulated with PDGF (2-6), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda (delta) phosphatase (6) or untreated (1-5), w


Requested From: 
Date Requested: 6/24/2018

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