Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
IHC - Paraffin
Western blot (1:1000)
Specificity and Use
Akt Substrate antibody was raised against synthetic phospho- Akt substrate peptides.
Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins.
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Western Blot: 1:1000. Immunoprecipitation 1:20. Immunohistochemistry (Paraffin): 1:100. ELISA: 1:1000.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, BSA, 50% glycerol.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.