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Anti-AES / Groucho Antibody (C-Terminus) IHC-plus™ LS-B441

Note: This antibody replaces LS-C19534, LS-C56769, LS-C56868, LS-C34910, LS-C42326

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AES / Groucho Antibodies

Anti-AES / Groucho Antibody (C-Terminus) IHC-plus™ LS-B441
Rabbit Polyclonal to Human AES / Groucho
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AES / Groucho Antibody (N-Terminus) IHC-plus™ LS-B1326
Rabbit Polyclonal to Human AES / Groucho
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AES / Groucho Antibody (aa79-91) IHC-plus™ LS-B8362
Goat Polyclonal to Human AES / Groucho
Human, Mouse, Rat, Bovine, Dog, Hamster, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human AES / Groucho
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human AES / Groucho
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Western blot (1 - 2 µg/ml)

Specificity and Use

AES / Groucho antibody was raised against synthetic peptide from human AES / GRG / Groucho.
LS-E30175 - Liquid - 50 µg - $145.00
C-Terminus
16 amino acid peptide from near the carboxy terminus of human AES
Immunohistochemistry: LS-B441 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B441 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About AES / Groucho

Q08117 NM_198969 NP_945320.1

AES Antibody, AES-1 Antibody, AES-2 Antibody, Amino enhancer of split Antibody, ESP1 Antibody, Groucho Antibody, GRG Antibody, Protein ESP1 Antibody, TLE5 Antibody, Protein GRG Antibody, gp130-associated protein GAM Antibody, GRG5 Antibody

Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.

Immunohistochemistry

Immunohistochemistry
Anti-AES antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B441 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of AES in HeLa cells with AES antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of AES in HeLa cells with AES antibody at 20 ug/ml.

Western blot

Western blot
Western blot of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

Requested From: United States
Date Requested: 12/11/2016

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