(tested or 100% immunogen sequence identity)
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ADGRE1 / EMR1 antibody was raised against cultured macrophages.
The antigen is a 125kD extracellular membrane protein sensitive to 2-mercaptoethanol. Antigen distribution: Isolated Cells: The antigen is expressed in vitro on over 80% of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes. Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin) except lung. It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas, the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells. Specificity: Mouse: major subpopulation of resident tissue macrophages Other species: human heart macrophages
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS pH 7.2, 5mg/ml BSA
500 µl Distilled water
Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.